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Sperm were exposed to THC for up to 24 h at ambient temperature in non-capacitating conditions and indirect effects on mitochondrial function were determine by computer-assisted sperm analyses ( A ) for quantification of total and progressive motility average-path velocity (VAP), and curvilinear velocity (VCL) at 0 and 24 h. Direct assessment of mitochondrial function was conducted using high-resolution sperm respirometry (O2K) for quantification of respirometry values ( B ) including: Basal, ATP-linked, ADP-dependent, Spare, Maximal, Complex I, Complex III and Proton Leak respiration. Data are presented as mean ± SEM. n = 3 bulls; 3 individual replicates/bull. Different superscripts ( a,b ) indicate P<0.05 between treatments.

Journal: bioRxiv

Article Title: Tetrahydrocannabinol exposure to postejaculatory sperm compromises sperm structure, function, the epigenome, and early embryo development

doi: 10.64898/2026.03.23.713385

Figure Lengend Snippet: Sperm were exposed to THC for up to 24 h at ambient temperature in non-capacitating conditions and indirect effects on mitochondrial function were determine by computer-assisted sperm analyses ( A ) for quantification of total and progressive motility average-path velocity (VAP), and curvilinear velocity (VCL) at 0 and 24 h. Direct assessment of mitochondrial function was conducted using high-resolution sperm respirometry (O2K) for quantification of respirometry values ( B ) including: Basal, ATP-linked, ADP-dependent, Spare, Maximal, Complex I, Complex III and Proton Leak respiration. Data are presented as mean ± SEM. n = 3 bulls; 3 individual replicates/bull. Different superscripts ( a,b ) indicate P<0.05 between treatments.

Article Snippet: Sperm kinematic parameters were objectively assessed by computer assisted sperm analysis (CASA-IVOS System, Hamilton Thorne, Beverly, MA) [ ].

Techniques: